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Updated: May 2, 2026

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Published on: May 26, 2014
Benzoselenadiazole-based responsive long-lifetime photoluminescent probes for protein kinases
Ramesh Ekambaram1, Erki Enkvist, Ganesh babu Manoharan
1Institute of Chemistry, University of Tartu, 14A Ravila St., 50411 Tartu, Estonia. asko.uri@ut.ee.
Abstract:
Benzoselenadiazole-containing inhibitors of protein kinases were constructed and their capability to emit phosphorescence in the kinase-bound state was established. Labelling of the inhibitors with a red fluorescent dye led to sensitive responsive photoluminescent probes for protein kinase CK2 that emitted red light with a long (microsecond-scale) decay time upon excitation of the probes with a pulse of near-UV light.
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