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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
Loss of CDC14B expression in clear cell renal cell carcinoma: meta-analysis of microarray data sets
Younghye Kim1, Jung-Woo Choi, Ju-Han Lee
1Dept of Pathology, Korea University Ansan Hospital, 516, Gojan-1 Dong, Danwon-Gu, Ansan-Si, Gyeonggi-Do, 425-707, Republic of Korea;
Objectives:
To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis.
Methods:
The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry.
Results:
CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B.
Conclusions:
Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
Insights
Microarray meta-analysis identified CDC14B as a significantly downregulated gene in clear cell renal cell carcinoma (ccRCC). Loss of CDC14B expression correlates with advanced disease stages and poorer recurrence-free survival in ccRCC patients.
Area of Science:
- Oncology
- Genetics
- Bioinformatics
Background:
- Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer.
- Identifying novel molecular markers is crucial for understanding ccRCC pathogenesis and progression.
Purpose of the Study:
- To identify novel differentially expressed genes (DEGs) in ccRCC using a meta-analysis approach.
- To investigate the clinical significance of the most downregulated gene in ccRCC.
Main Methods:
- Meta-analysis of five ccRCC microarray datasets using a random-effects model.
- Validation of the most downregulated gene (CDC14B) by immunohistochemistry in 80 paired ccRCC tissues.
Main Results:
- CDC14B was identified as the most significantly downregulated gene among 1,761 DEGs.
- Loss of CDC14B expression was observed in 87.5% of ccRCC cases.
- Complete loss of CDC14B correlated with advanced T stage, TNM stage, tumor recurrence, and shorter recurrence-free survival.
Conclusions:
- Microarray meta-analysis is a valuable tool for discovering DEGs in ccRCC.
- Downregulation of CDC14B suggests its role as a potential tumor suppressor in renal carcinogenesis.
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