[Study on quality standard of Chimonanthus nitens leaf]

To set up the quality standard of Chimonanthus nitens leaf.

Five compounds were identified by double-development thin layer chromatography, the plate was colorized with 1% vanillin-H2 SO4 solution and warmed up in 110 degrees C; The content of four compounds was determined by HPLC, the chromatography separation was performed on the Elite hypersil ODS2 (250 mm x 4.6 mm, 5 microm) column with gradient elution. The mixture of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min was used, the detection wavelength was 344 nm and the column temperature was set at 40 degrees C.

The TLC identification was highly specific and spots were clear. In HPLC method, the calibration curve was linear in the range of 5.665 - 56.65 microg/mL for scopoletin (r = 0.9999), the average recovery was 97.06% and RSD was 1.27%; The calibration curve was linear in the range of 2.4312 - 24.312 microg/mL for isofraxidin (r = 0.9999), the average recovery was 102.86% and RSD% was 0.93%; The calibration curve presented a linear range from 2.444 - 24.44 microg/mL for scoparone (r = 0.9999), the average recovery was 102.18% and RSD was 1.81%. The standard curve presented a linear range from 9.012 - 90.12 microg/mL for rutin (r = 0.9992), the average recovery was 104.44% and RSD was 4.2%.

These methods are reproducible, sensitive and simple and can be used to control the quality of Chimonanthus nitens leaf.