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Updated: May 2, 2026

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
A new cytotoxic steroid from co-fermentation of two marine alga-derived micro-organisms
Sherif S Ebada1, Thomas Fischer, Sarah Klaßen
1a BIO-MAR , Merowingerplatz 1a, D-40225 Düsseldorf , Germany.
Abstract:
Bioactivity-guided chemical investigation of a co-culture of marine-derived micro-organisms has yielded one new steroid, 7β-hydroxycholesterol-1β-carboxylic acid (1) with an unprecedented carboxylic acid group at C-1, together with three known steroidal metabolites (2-4). The chemical structures and stereochemistry of the isolated compounds were unambiguously determined based on extensive 1D, 2D NMR and HR-ESI-MS measurements. The isolated compounds were assessed for their cytotoxic activity against four different human tumour cell lines K562 (leukaemia), HCT116 (colon), A2780 (ovary) and its cisplatin-resistant mutant (A2780 CisR), and they revealed moderate activities with IC50 values ranging from 10.0 to 100.0 μM.
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