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Porphyromonas gingivalis outer membrane vesicles exclusively contain outer membrane and periplasmic proteins and

Paul D Veith1, Yu-Yen Chen, Dhana G Gorasia

  • 1Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne , 720 Swanston Street, Victoria, 3010, Australia.

Journal of Proteome Research
|March 14, 2014
PubMed

Insights

Porphyromonas gingivalis releases virulence factors via outer membrane vesicles (OMVs). These OMVs are enriched with specific proteins, aiding pathogen survival and disease progression in chronic periodontitis.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Proteomics

Background:

  • Porphyromonas gingivalis is a key pathogen in chronic periodontitis.
  • The bacterium releases outer membrane vesicles (OMVs) containing virulence factors.

Purpose of the Study:

  • To characterize the proteome of P. gingivalis OMVs.
  • To understand the mechanism of virulence factor enrichment in OMVs.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze OMV proteins.
  • Cryo-transmission electron microscopy (Cryo-TEM) was employed to visualize OMV structure.

Main Results:

  • 151 OMV proteins were identified, primarily from the outer membrane or periplasm.
  • Proteins with C-terminal domains (CTD) were enriched on the OMV surface.
  • Specific proteins like OmpA and TonB-dependent receptors were retained on the bacterial cell.

Conclusions:

  • P. gingivalis selectively packages virulence factors into OMVs.
  • CTD proteins contribute significantly to the OMV structure and enrichment.
  • OMVs play a crucial role in delivering virulence factors during infection.

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