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Updated: May 2, 2026

Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
Autogenous bone grafts contamination after exposure to the oral cavity
Hugo Nary Filho1, Tábata Fernandes Pinto, Caio Peixoto de Freitas
1From the *Department of Oral Biology Post Graduation, and †Dentistry Course, University do Sagrado Coração (USC), Bauru, São Paulo, Brazil.
Abstract:
The purpose of this paper was to analyze specimens of autogenous bone block grafts exposed to the oral cavity after ridge reconstructions. Specimens of chronic suppurative osteomyelitis (CSO) of the jaws were used as comparison for bacterial colonization pattern. For this, 5 specimens of infected autogenous bone grafts were used and 10 specimens of CSO embedded in paraffin were stained with Brown and Brenn technique and analyzed under light microscopy. The results showed a similar colonization pattern in both situations, with the establishment of bacterial biofilm and the predominance of Gram-positive bacteria. The conclusion was that the similarity in bacterial distribution and colonization between autogenous bone grafts and CSO stresses the necessity of more invasive procedures for the treatment of the autogenous bone grafts early exposed to the oral cavity.
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