Related Experiment Video
Updated: May 2, 2026
10:55
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Published on: October 16, 2018
7.1K
Cell surface vimentin is an attachment receptor for enterovirus 71
Ning Du1, Haolong Cong, Hongchao Tian
1Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Journal of Virology
|March 14, 2014
Summary
Enterovirus 71 (EV71) uses vimentin on cell surfaces for attachment, increasing infection. Blocking this interaction with soluble vimentin or antibodies offers new treatment strategies for EV71 diseases.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Enterovirus 71 (EV71) causes severe central nervous system diseases in infants and children.
- Understanding EV71's cellular entry mechanisms is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the role of vimentin intermediate filaments in EV71 infection.
- To identify potential therapeutic targets for EV71 pathogenesis.
Main Methods:
- Investigated the binding of EV71 VP1 protein to host cell surface vimentin.
- Utilized soluble vimentin and anti-vimentin antibodies to block binding.
- Assessed the impact of reduced cell surface vimentin expression on EV71 attachment.
Main Results:
- EV71 VP1 protein directly binds to vimentin intermediate filaments on the host cell surface.
- Soluble vimentin and antibodies against vimentin inhibit EV71 binding.
- Reduced vimentin expression on the cell surface significantly decreased EV71 attachment.
- The N terminus of vimentin mediates the interaction with EV71.
Conclusions:
- Vimentin on the host cell surface acts as an attachment site for EV71.
- This interaction facilitates initial binding and enhances EV71 infectivity.
- Targeting the vimentin-EV71 interaction presents a promising therapeutic strategy for EV71 infections.
Related Concept Videos
01:38Intralumenal Vesicles and Multivesicular Bodies
4.0K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.0K
01:10Intracellular Movement of Viruses and Bacteria
3.0K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.0K
01:18Viral Meningitis
199
Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...
199
01:18Surface Membrane Barriers
3.0K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
3.0K
01:24Adherens Junctions
5.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
5.8K
01:31Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K