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Demonstration of immunochemical identity between the synaptic vesicle proteins synaptin and synaptophysin/p38
H Gaardsvoll1, D Obendorf, H Winkler
1Research Center for Medical Biotechnology, University of Copenhagen, Denmark.
FEBS Letters
|December 19, 1988
Abstract:
The synaptic vesicle proteins synaptin and synaptophysin/p38 were shown to be immunochemically identical. Western immunoblot analysis of Triton X-100 extracts from rat brain showed that polyclonal polyspecific anti-synaptin antibodies and monoclonal antibody SY38 against synaptophysin both reacted with a band of 38 kDa. In two-dimensional immunoblots of chromaffin granule membranes from bovine adrenal medulla anti-synaptin and anti-synaptophysin antibodies also recognized the same component. Finally, in a Western immunoblotting experiment SY38 reacted with an immuno-isolated synaptin antigen.