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Sodium influxes in renal epithelial LLC-PK1/Cl4 cells monitored by 23Na NMR
A W Jans1, R Willem, E R Kellenbach
1Max-Planck-Institut fuer Systemphysiologie, Dortmund, Federal Republic of Germany.
Magnetic Resonance in Medicine
|July 1, 1988
Abstract:
23Na NMR experiments with a suspension of the renal epithelial cell line, LLC-PK1/Cl4, in the presence of the shift reagent dysprosium polyphosphate showed that the intracellular sodium was only NMR visible for 64 +/- 4%. Intracellular sodium content was found to be 30.6 +/- 1.2 mM (25 degrees C). Examination of the sodium influx during recovery from intracellular acidification showed that sodium is transported not only by Na+/H+ exchange but also by sodium-D-glucose cotransport with a stoichiometry of 1:1.