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Updated: May 2, 2026

Assessing Whole-Body Lipid-Handling Capacity in Mice
Published on: November 24, 2020
Lipemia: causes, interference mechanisms, detection and management.
1University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice, Zagreb, Croatia.
Lipemia, a common interference in clinical labs, can cause errors. Proper sample handling and lipid removal protocols are crucial for accurate patient test results and safety.
Area of Science:
- Clinical Chemistry
- Laboratory Medicine
- Analytical Chemistry
Background:
- Lipemia is a frequent endogenous interference in clinical laboratory testing, second only to hemolysis.
- It can significantly impact various laboratory methods due to preanalytical factors like postprandial sampling or lipid emulsions.
- Detecting lipemia via lipemic index has limitations, including false positives and lack of standardization.
Purpose of the Study:
- To highlight the challenges posed by lipemia in laboratory diagnostics.
- To emphasize the need for standardized protocols for lipemia detection and interference removal.
- To advocate for revised acceptance criteria for lipemia interference based on biological variation.
Main Methods:
- Review of common preanalytical causes of lipemic samples.
- Discussion of analytical platforms for lipemic index measurement and their limitations.
- Exploration of methods for removing lipemia interference from samples.
- Analysis of manufacturer data verification and acceptance criteria.
Main Results:
- Lipemia can be removed from samples, allowing for accurate measurements, but requires careful protocol selection based on analytes.
- Existing methods for detecting lipemia and manufacturer-reported data verification show significant limitations.
- Current acceptance criteria for lipemia interference are not based on biological variation and require revision.
Conclusions:
- Standardized procedures for detecting, removing, and reporting lipemic sample results are essential.
- Clear protocols improve accuracy, reduce errors, and enhance patient safety in clinical laboratories.
- Manufacturers must improve verification of lipemia interference data and align acceptance criteria with biological variation.
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