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Updated: May 2, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
[Gut microbiota may have influence on glucose and lipid metabolism]
Kristian Hallundbæk Mikkelsen1, Morten Frost Nielsen, Michael Tvede
1Diabetologisk Forskningsenhed, Medicinsk Afdeling F, Gentofte Hospital, Niels Andersens Vej 65, 2900 Hellerup. kristianmikkel@gmail.com
Abstract:
New gene sequencing-based techniques and the large worldwide sequencing capacity have introduced a new era within the field of gut microbiota. Animal and human studies have shown that obesity and type 2 diabetes are associated with changes in the composition of the gut microbiota and that prebiotics, antibiotics or faecal transplantation can alter glucose and lipid metabolism. This paper summarizes the latest research regarding the association between gut microbiota, diabetes and obesity and some of the mechanisms by which gut bacteria may influence host metabolism.
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