Related Experiment Video
Updated: May 2, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Indirect photometric detection of boron cluster anions electrophoretically separated in methanol
Lada Vítová1, Lukáš Fojt1, Radim Vespalec1
1Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 35 Brno, Czech Republic.
Abstract:
3,5-Dinitrobenzoate and picrate are light absorbing anions pertinent to indirect photometric detection of boron cluster anions in buffered methanolic background electrolytes (BGEs). Tris(hydroxymethyl)aminomethane and morpholine have been used as buffering bases, which eliminated baseline steps, and minimized the baseline noise. In methanolic BGEs, mobilities of boron cluster anions depend on both ionic constituents of the BGE buffer. This dependence can be explained by ion pair interaction of detected anions with BGE cations, which are not bonded into ion pairs with the BGE anions. The former ion pair interaction decreases sensitivity of the indirect photometric detection.
Related Concept Videos
Hydroboration-Oxidation of Alkenes
Gas Chromatography: Types of Detectors-II
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
High-Performance Liquid Chromatography: Types of Detectors
Chemical Ionization (CI) Mass Spectrometry
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

