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Updated: May 2, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of
Caroline Bylda1, Roland Thiele, Uwe Kobold
1Roche Diagnostics GmbH, Penzberg, Germany.
Abstract:
Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.
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