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Updated: May 2, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation
Joshua P Ferreira1, William L Noderer1, Alexander J Diaz de Arce1
1Department of Chemical Engineering; Stanford University; Stanford, CA USA.
Abstract:
We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.
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