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The do's and don'ts of effectoromics
Juan Du1, Vivianne G A A Vleeshouwers
1Wageningen UR Plant Breeding, Wageningen University & Research Centre, 386, 6700 AJ, Wageningen, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|March 20, 2014
Summary
Effectoromics, a functional genomics method, accelerates plant R gene discovery and deployment in breeding. This study offers guidance and protocols for applying effectoromics assays in crops like potato.
Area of Science:
- Plant science
- Genomics
- Agricultural science
Background:
- Effectoromics is a high-throughput functional genomics approach for identifying plant resistance (R) genes.
- It aids modern resistance breeding by probing plant germplasm with effectors.
- Key advantages include accelerated R gene identification, distinguishing functional redundancy, detecting recognition specificity, and assisting R gene deployment.
Purpose of the Study:
- To provide practical suggestions and reminders for applying effectoromics in plant breeding.
- To present and explain two routine functional assays: agroinfiltration and agroinfection.
- To offer protocols for these assays in model systems like Nicotiana benthamiana and crops such as potato (Solanum tuberosum).
Main Methods:
- Utilizing effectoromics, a functional genomics technique.
- Employing agroinfiltration and agroinfection assays.
- Applying protocols in Nicotiana benthamiana and Solanum tuberosum.
Main Results:
- Effectoromics offers significant advantages for resistance breeding.
- Agroinfiltration and agroinfection are presented as key functional assays.
- Protocols are provided for applying these methods in model and crop species.
Conclusions:
- Effectoromics is a powerful tool for enhancing plant resistance breeding strategies.
- Standardized protocols for key assays facilitate broader application.
- This approach aids in the efficient identification and deployment of R genes for improved crop resilience.
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