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Differential splicing of MAG transcripts during CNS and PNS development
M B Tropak1, P W Johnson, R J Dunn
1Division of Molecular Immunology and Neurobiology, Mt. Sinai Hospital Research Institute, Toronto, Ont., Canada.
Brain Research
|September 1, 1988
Summary
Two messenger RNA (mRNA) variants of myelin-associated glycoprotein (MAG) are generated by alternative splicing, leading to distinct protein forms (p67MAG and p72MAG) with differential developmental regulation in the central and peripheral nervous systems.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Myelin-associated glycoprotein (MAG) is crucial for glial-neuronal adhesion during myelination.
- Previous studies predicted two MAG mRNA classes differing by a 45-nucleotide insert.
Purpose of the Study:
- To confirm the existence and origin of two MAG mRNA variants.
- To investigate the differential expression and regulation of MAG isoforms during nervous system development.
Main Methods:
- RNAse protection assays were used to analyze MAG mRNA expression.
- cDNA sequencing was employed to identify MAG mRNA variants.
Main Results:
- Two MAG mRNAs, differing by alternative splicing of exon 12, were confirmed.
- p72MAG mRNA peaks during CNS myelin formation, while p67MAG mRNA predominates in adult brain.
- p67MAG mRNA is dominant in the PNS throughout development, with p72MAG mRNA being a minor species.
- Alternative splicing at the 5' terminus also contributes to MAG mRNA diversity, with distinct CNS and PNS patterns.
Conclusions:
- The two MAG protein forms (p67MAG and p72MAG) are generated by alternative splicing of MAG mRNA.
- Differential developmental regulation of MAG isoforms occurs in both the central nervous system (CNS) and peripheral nervous system (PNS).