Related Experiment Video
Updated: Apr 22, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
The production of monoclonal antibodies from human lymphocytes
1Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Two conceptually unique approaches are currently available for the production of human monoclonal antibodies - the 'hybridoma' technique, based on the fusion of antibody-producing B lymphocytes with plasmacytoma cells or lymphoblastoid cell lines; and the use of Epstein-Barr virus (EBV) to 'immortalize' antigen-specific, human B lymphocytes. Here Danuta Kozbor and, John Roder discuss the contribution each technique is making, alone or in combination, towards the desired aim of producing human monoclonal antibodies with a defined specificity.
Two conceptually unique approaches are currently available for the production of human monoclonal antibodies - the 'hybridoma' technique, based on the fusion of antibody-producing B lymphocytes with plasmacytoma cells or lymphoblastoid cell lines; and the use of Epstein-Barr virus (EBV) to 'immortalize' antigen-specific, human B lymphocytes. Here Danuta Kozbor and, John Roder discuss the contribution each technique is making, alone or in combination, towards the desired aim of producing human monoclonal antibodies with a defined specificity.
