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Updated: Jul 3, 2026
A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
Published on: August 13, 2009
[Establishment of localization ultrathin section for cytopathic cells]
Objective:
To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.
Methods:
Lab-Tek Chamber slide system (177402) was selected as resin embedding mould. Cells infected with Human adenovirus type 5 (Ad5) or A/HN/SWL3/ 2009 (H1N1) influenza virus were embedded in situ as models. Target cytopathic cells were exposed by trimming, sectioned and observed under transmission electron microscope (TEM).
Results:
Target cells could be sectioned in situ and virus particles could be found easily on sections.
Conclusion:
A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.
