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Updated: May 2, 2026

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
Published on: December 21, 2016
Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking
Hong-Kee Tan1, Cheng-Xu Delon Toh, Dongrui Ma
1Epigenetics and Cell Fates Laboratory, A*STAR Institute of Molecular and Cell Biology, Singapore; Research and Development Unit, National Heart Centre Singapore, Singapore; Stem Cell and Developmental Biology, Genome Institute of Singapore, and Bioinformatics Institute, A*STAR, Singapore; Center for Individualized Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota, USA; Health Sciences Authority, Singapore; Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore; Department of Paediatrics, University Children's Medical Institute, National University Hospital, Singapore; Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA; Department of Biochemistry, Yong Loo Lin School of Medicine, Department of Biological Sciences, and NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore; Duke-NUS Graduate Medical School, Singapore; Royal Brompton Hospital, London, United Kingdom; National Heart and Lung Institute, Imperial College, London, United Kingdom.
Abstract:
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.
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