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miR-141 is a key regulator of renal cell carcinoma proliferation and metastasis by controlling EphA2 expression

Xuanyu Chen1, Xuegang Wang1, Anming Ruan1

  • 1Authors' Affiliations: Department of Urology, Union Hospital; Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Cancer Biology Program, Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts; and Department of Neurology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Georgia Regents University, Augusta, Georgia.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|March 21, 2014
PubMed
Abstract

Insights

MicroRNA-141 (miR-141) is significantly downregulated in clear cell renal cell carcinoma (ccRCC). Restoring miR-141 suppresses ccRCC growth and metastasis by targeting EphA2 signaling.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • MicroRNAs (miRNAs) are key regulators of tumorigenesis.
  • Understanding miRNA roles in renal cell carcinoma (RCC) is crucial for developing targeted therapies.

Purpose of the Study:

  • Identify human miRNAs regulating renal carcinogenesis.
  • Investigate the function of miR-141 in clear cell RCC (ccRCC).

Main Methods:

  • Microarray profiling of ccRCC and normal tissues.
  • In vitro and in vivo functional studies of miR-141.
  • Analysis of miR-141 expression correlation with EphA2 in human ccRCC samples.

Main Results:

  • 74 miRNAs were dysregulated in ccRCC; miR-141 was significantly downregulated.
  • miR-141 acts as a biomarker for ccRCC detection (AUC=0.93).
  • Overexpression of miR-141 inhibited ccRCC cell proliferation, migration, and invasion via the EphA2/FAK/AKT/MMP2/9 pathway.

Conclusions:

  • miR-141 is a potential biomarker for ccRCC diagnosis.
  • miR-141 functions as a tumor suppressor in ccRCC by inhibiting proliferation and metastasis.
  • The EphA2/p-FAK/p-AKT/MMPs signaling cascade is modulated by miR-141 in ccRCC.

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