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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

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Related Experiment Video

Updated: May 2, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Transcriptome analysis reveals differential splicing events in IPF lung tissue.

Tracy Nance¹, Kevin S Smith¹, Vanessa Anaya¹

¹Department of Pathology, Stanford University, Stanford, California, United States of America.

|March 21, 2014

Summary

Idiopathic pulmonary fibrosis (IPF) involves significant gene expression and splicing changes. RNA sequencing revealed altered gene activity and alternative splicing in IPF lung tissue, suggesting new disease mechanisms.

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Area of Science:

Molecular Biology
Genomics
Pulmonary Medicine

Background:

Idiopathic pulmonary fibrosis (IPF) is a complex lung disease with poorly understood molecular underpinnings.
Traditional methods like microarrays have limitations in detecting alternative splicing events.

Purpose of the Study:

To investigate differential gene expression and alternative splicing in IPF using RNA sequencing.
To identify novel molecular pathways and potential therapeutic targets in IPF.

Main Methods:

RNA sequencing (RNA-Seq) of messenger RNA from 8 IPF lung samples and 7 healthy controls.
Bioinformatic analysis to identify differentially expressed genes and differentially spliced genes.
Quantitative PCR (qPCR) for validation of specific splicing events.

Main Results:

Identified 873 differentially expressed genes (FDR<5%) and 440 genes with significant differential splicing (FDR<5%) in IPF lungs.
Validated differential exon usage in COL6A3 and POSTN genes, encoding extracellular matrix proteins.
Observed altered splicing of collagen and periostin, with periostin's differential splicing being a novel finding in IPF.

Conclusions:

Alternative splicing of genes, including COL6A3 and POSTN, may play a significant role in IPF pathogenesis.
RNA-Seq provides a powerful tool for uncovering complex genetic alterations in IPF.
An interactive web application was developed to facilitate further research into IPF gene regulation.