Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D printing in core facilities - Low pain, high gain.

Journal of microscopy·2026
Same author

Stress and conflict management in core facilities-News from an imaging survey.

Journal of microscopy·2026
Same author

Quantitative Mapping of Nanoscale EGFR-Grb2 Assemblies by DNA-PAINT.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.

Angewandte Chemie (International ed. in English)·2026
Same author

Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.

ACS nano·2026
Same author

Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.

Journal of the American Chemical Society·2025
Same journal

Altered expression of Toll-like receptor 9 in the lung tissue of adult mice generated by in vitro embryo culture and embryo transfer.

Histochemistry and cell biology·2026
Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biology·2026
Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biology·2026
Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
Same journal

Immunohistochemical study of α-keratin, loricrin, filaggrin-like protein, and transglutaminase-1 expression in orthokeratinized and parakeratinized epithelium of the tongue of domestic goose (Anser anser f. domestica) during embryonic development.

Histochemistry and cell biology·2026
See all related articles

Related Experiment Video

Updated: May 2, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775

Integrated and correlative high-throughput and super-resolution microscopy.

Manuel Gunkel1, Benjamin Flottmann, Mike Heilemann

  • 1BioQuant Centre, Heidelberg University, INF 267, 69120, Heidelberg, Germany, manuel.gunkel@bioquant.uni-heidelberg.de.

Histochemistry and Cell Biology
|March 21, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method for multi-scale microscopy experiments. This technique combines low-resolution screening with super-resolution microscopy to analyze cellular phenotypes with high precision.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K
Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

773

Related Experiment Videos

Last Updated: May 2, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K
Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

773

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biophysics

Background:

  • Investigating cellular phenotypes often requires multi-scale imaging approaches.
  • Combining low-resolution and super-resolution microscopy can provide comprehensive cellular insights.
  • Current methods may lack efficient strategies for correlating data across different scales.

Purpose of the Study:

  • To develop and validate a novel method for multi-scale microscopic experiments.
  • To enable seamless integration of low-resolution screening with super-resolution imaging.
  • To facilitate detailed analysis of cellular phenotypes at multiple spatial and temporal scales.

Main Methods:

  • Utilized a low-resolution, fast data acquisition screen for phenotypic cellular responses.
  • Employed feature space analysis for automatic selection of cells with characteristic alterations.
  • Implemented coordinate transfer using fixed cells as reference points for super-resolution imaging on a dSTORM microscope.
  • Avoided the need for additional fiducial markers in coordinate transfer.

Main Results:

  • Successfully demonstrated a method for multi-scale cellular phenotype analysis.
  • Enabled automatic identification and selection of relevant cellular populations from large screening datasets.
  • Achieved accurate coordinate transfer between low-resolution and super-resolution microscopy datasets.
  • Showcased the compatibility of the method with complementary fluorescence microscopy systems.

Conclusions:

  • The developed method allows for efficient multi-scale imaging of cellular phenotypes.
  • This approach enhances the ability to gain deeper insights into biological specimens.
  • The technique is versatile and can be applied to combine various fluorescence microscopy techniques for multi-scale analysis.