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Updated: May 2, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
Gene expression profiling of sense and antisense transcripts in liver regeneration by microarray analysis.
Mitsuru Chiba1, Hiroshi Yasue2, Nobuhiro Ohkohchi3
1Department of Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575; ; Department of Biomedical Sciences, Division of Medical Life Sciences, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036-8564;
This study identifies novel sense and antisense transcripts involved in liver regeneration after partial hepatectomy in mice. These findings advance our understanding of gene regulation during this critical biological process.
Area of Science:
- Molecular Biology
- Genetics
- Hepatology
Background:
- Liver regeneration is a complex process involving numerous genes.
- Natural antisense transcripts regulate gene expression, but their role in liver regeneration is unknown.
Purpose of the Study:
- To comprehensively identify sense and antisense transcripts that are up- or downregulated during liver regeneration.
- To investigate the role of these transcripts in the regenerative process.
Main Methods:
- Utilized partial hepatectomy (PH) in mice to induce liver regeneration.
- Employed a custom sense/antisense microarray to analyze transcript expression.
- Validated key findings using strand-specific reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
Main Results:
- Identified 97 upregulated and 7 downregulated sense transcripts.
- Discovered 15 upregulated and 2 downregulated antisense transcripts in regenerating livers.
- Observed concordant upregulation of sense and antisense transcripts for genes like Apoa4, Hp, Fgb, and Fgg.
Conclusions:
- The study identified novel sense and antisense transcripts implicated in liver regeneration.
- These findings suggest a regulatory role for antisense transcripts in liver regeneration.
- The identified transcripts provide potential targets for further research into liver repair mechanisms.
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