Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Erythropoiesis01:14

Erythropoiesis

5.7K
Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
5.7K
Erythropoiesis01:14

Erythropoiesis

4.0K
4.0K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

2.3K
2.3K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Curbing Unnecessary Vitamin D Testing.

NEJM catalyst innovations in care delivery·2026
Same author

Early postnatal DNA methylation dynamics define neuronal subtypes and are disrupted by MECP2 loss.

bioRxiv : the preprint server for biology·2026
Same author

Prognostic and therapeutic implications of BRAF mutations in acute myeloid leukemia.

Leukemia·2026
Same author

Integrated proteogenomic and metabolomic profiling of acute myeloid leukemias to identify molecular subtypes and associated therapy targets.

Nature cancer·2026
Same author

A multi-center study on the consistency of drug sensitivity testing in patients with acute myeloid leukemia.

NPJ precision oncology·2026
Same author

Results of a phase 1 trial testing ruxolitinib plus venetoclax in patients with relapsed/refractory acute myeloid leukemia.

Blood neoplasia·2026

Related Experiment Video

Updated: May 2, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

32.9K

Corepressor Rcor1 is essential for murine erythropoiesis.

Huilan Yao1, Devorah C Goldman2, Tamilla Nechiporuk1

  • 1Vollum Institute.

Blood
|March 22, 2014
PubMed
Summary
This summary is machine-generated.

The corepressor Rcor1 is essential for red blood cell development. Its absence causes anemia and myeloid cell differentiation in erythroid progenitors, revealing Rcor1

More Related Videos

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

14.6K
Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
11:46

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Published on: December 14, 2018

6.0K

Related Experiment Videos

Last Updated: May 2, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

Published on: August 5, 2011

32.9K
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
06:40

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

14.6K
Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
11:46

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Published on: December 14, 2018

6.0K

Area of Science:

  • Hematopoiesis
  • Molecular Biology
  • Developmental Biology

Background:

  • The corepressor Rcor1's role in hematopoiesis is biochemically suggested but functionally uncharacterized in vivo.
  • Understanding Rcor1's in vivo function is crucial for elucidating mechanisms of blood cell development.

Purpose of the Study:

  • To investigate the in vivo function of the corepressor Rcor1 in hematopoiesis.
  • To determine the cellular and molecular consequences of Rcor1 deletion during erythropoiesis.

Main Methods:

  • Generation and analysis of Rcor1-deleted mice.
  • In vitro culture of erythroid progenitors.
  • Gene expression analysis (including myeloid and HSC/progenitor genes).
  • Analysis of colony-stimulating factor 2 receptor β subunit (Csf2rb) regulation and Stat5 signaling.

Main Results:

  • Rcor1 deletion in mice leads to severe anemia and embryonic lethality.
  • Erythroid cells from Rcor1-null mice arrest during development and aberrantly differentiate into myeloid colonies in vitro.
  • Mutant proerythroblasts express myeloid lineage genes and genes associated with hematopoietic stem cells (HSCs) and progenitor cells.
  • Csf2rb is a direct target of Rcor1 and Gfi1b; its absence induces Csf2rb expression and Stat5 signaling hypersensitivity, promoting myeloid fate.

Conclusions:

  • Rcor1 is indispensable for erythropoiesis, acting to suppress HSC/progenitor genes and myeloid differentiation pathways.
  • Rcor1 maintains erythroid lineage commitment by repressing alternative cell fates.
  • The findings highlight Rcor1's critical role in preventing aberrant myeloid differentiation during red blood cell development.