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Updated: May 2, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Published on: September 10, 2017
A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy
Eiji Kobayashi1, Hiroyuki Kishi1, Atsushi Muraguchi1
1Department of Immunology; Graduate School of Medicine and Pharmaceutical Sciences; University of Toyama; Toyama, Japan.
Abstract:
T-cell receptor (TCR)-based gene immunotherapy has emerged as a promising approach for the treatment of multiple malignancies. We have recently reported an efficient system for the cloning and functional evaluation of TCR-coding cDNAs. This system, which we named hTEC10, allows for the determination of TCR antigen specificity in less than 10 days, and may therefore constitute a fast and powerful platform for the development of new TCR-based anticancer therapies.
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