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Updated: May 1, 2026
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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
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Immunology of psoriasis
Michelle A Lowes1, Mayte Suárez-Fariñas, James G Krueger
1Laboratory for Investigative Dermatology, The Rockefeller University, New York, NY 10065; email: lowesm@rockefeller.edu , farinam@rockefeller.edu , jgk@rockefeller.edu.
Annual Review of Immunology
|March 25, 2014
Summary
Psoriasis involves skin immune cells releasing cytokines like IL-23 and IL-12, activating T cells to produce inflammatory cytokines. Therapies targeting these cytokines show promise for treating this common skin disease.
Area of Science:
- Dermatology
- Immunology
- Genetics
Background:
- The skin's immune system, including skin-associated lymphoid tissue (SALT), defends against injury and maintains homeostasis.
- Psoriasis vulgaris is a well-studied T cell and dendritic cell-mediated inflammatory skin disease.
Purpose of the Study:
- To elucidate the immune pathways and genetic factors involved in psoriasis vulgaris.
- To explore the relationship between psoriasis, comorbidities, and the skin transcriptome.
Main Methods:
- Analysis of cytokine production by myeloid dendritic cells and T cells (IL-17, Th1, Th22).
- Review of genetic factors including mutations, PSORS loci, and single nucleotide polymorphisms.
- Correlation of skin transcriptome and serum proteins to assess comorbidities.
- Examination of psoriasis-related cytokine pathways in mouse models.
Main Results:
- Inflammatory myeloid dendritic cells release IL-23 and IL-12, activating T cells to produce key psoriatic cytokines (IL-17, IFN-γ, TNF, IL-22).
- These cytokines amplify inflammation by affecting keratinocytes.
- Therapeutic targeting of IL-23, TNF, and IL-17 is effective.
Conclusions:
- Understanding the genetic basis and immune response in psoriasis is crucial for developing effective treatments.
- The skin transcriptome and serum proteome provide insights into psoriasis comorbidities.
- This research offers a model for studying other inflammatory skin and autoimmune diseases.
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