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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: May 1, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

A reductive ligation based fluorescent probe for S-nitrosothiols.

Di Zhang¹, Wei Chen, Zhengrui Miao

¹Department of Chemistry, Washington State University, Pullman, WA 99164, USA. mxian@wsu.edu.

Chemical Communications (Cambridge, England)

|March 25, 2014

Summary

This summary is machine-generated.

A new fluorescent probe was created to detect S-nitrosothiols (SNO). This probe demonstrates high selectivity and sensitivity for identifying SNO in samples.

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Area of Science:

Analytical Chemistry
Biochemistry
Chemical Biology

Background:

S-nitrosothiols (SNO) are crucial signaling molecules involved in various physiological and pathological processes.
Accurate detection of SNO is essential for understanding their biological roles and developing therapeutic strategies.
Existing methods for SNO detection often face limitations in sensitivity, selectivity, or operational simplicity.

Purpose of the Study:

To develop a novel fluorescent probe for the sensitive and selective detection of S-nitrosothiols (SNO).
To characterize the performance of the developed probe in terms of selectivity, sensitivity, and response mechanism.

Main Methods:

Design and synthesis of a novel fluorescent probe based on reductive ligation.
Evaluation of the probe's spectral properties and response to SNO.
Assessment of the probe's selectivity against various reactive sulfur and nitrogen species.
Testing the probe's performance in relevant biological matrices (if applicable).

Main Results:

A novel reductive ligation-based fluorescent probe was successfully synthesized.
The probe exhibited a distinct fluorescence response upon reaction with SNO.
The developed probe demonstrated excellent selectivity for SNO over other tested analytes.
High sensitivity was achieved, allowing for the detection of low concentrations of SNO.

Conclusions:

The developed fluorescent probe provides a robust tool for SNO detection.
This probe offers advantages in selectivity and sensitivity compared to existing methods.
The probe has potential applications in biological research and diagnostics for monitoring SNO levels.