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Cell sedimentation with gravity activation.

G Czerlinski1, R Goldman-Leikin, D Reid

  • 1Northwestern University Medical School, Chicago, IL 60611.

Cell Biophysics
|December 1, 1988
PubMed
Summary
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Murine monoclonal antibody T101 was attached to heavy alloy particles and then to T-cell leukemia cells. Researchers calculated approximately 33 particles per cell, with a surface saturation of 144 particles/cell.

Area of Science:

  • Biotechnology
  • Materials Science
  • Oncology

Background:

  • Murine monoclonal antibody T101 targets T-cells.
  • Heavy alloy particles (LaMn2Ge2) offer unique physical properties.
  • T-cell leukemia cell line RPMI 8402 (T8402) is a model for leukemia research.

Purpose of the Study:

  • To conjugate T101 antibody-bound particles to T-cell leukemia cells.
  • To quantify the number of particles attached to each cell.
  • To determine the surface saturation capacity of the cells for these particles.

Main Methods:

  • Coupling of T101 antibody to polymer-coated LaMn2Ge2 particles.
  • Attachment of antibody-particle conjugates to T8402 cells.
  • Measurement of sedimentation velocities for cells, particles, and conjugates.

Related Experiment Videos

  • Calculation of mean particle radii and number of particles per cell.

    • Main Results:

    • A mean of 33 T101-bound particles were calculated to attach to each T8402 cell.
    • Surface saturation was estimated at 144 particles per cell.
    • The study considered particle and cell radii ranging from 0.01 to 1 micron and 4 to 10 microns, respectively.

    Conclusions:

    • The study successfully quantified particle attachment to leukemia cells.
    • Findings provide insights into particle-cell conjugation for potential therapeutic or diagnostic applications.
    • Theoretical calculations support experimental observations on particle loading and saturation.