Analysis of platelet glycoproteins in thrombocytopathias using the lectin-avidin-biotin-peroxidase (LABP) technique

The application of the lectin-avidin-biotin-peroxidase (LABP) technique for detecting platelet glycoprotein abnormalities in thrombocytopathias is described. Platelet proteins from patients with Glanzmann's thrombasthenia or Bernard-Soulier syndrome were separated by two-dimensional O'Farrell gel electrophoresis, stained with silver or electroblotted onto nitrocellulose sheets. Nitro-cellulose blots were stained utilizing the LABP technique. The absence or severe reduction of glyco-proteins IIb and IIIa and fibrinogen in the platelet protein pattern of patients with thrombasthenia as well as the absence or marked reduction of glycoproteins Ib and V in the platelet protein pattern of a patient with Bernard-Soulier syndrome were clearly demonstrated.