Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid and Accurate Quantification of RNA in Lipid Nanoparticles by Scatter-Free UV/Visible Spectroscopy.

Nano letters·2025
Same author

Morphological Control of Y6 Thin Films Reveals Charge Transfer Is Facilitated by Co-facial Interactions.

The journal of physical chemistry letters·2025
Same author

Forward-Scattering and Multiple-Scattering Sources of Errors in UV-Visible Spectroscopy of Microspheres.

Analytical chemistry·2024
Same author

The importance of the simulated wound fluid composition and properties in the determination of the fluid handling performance of wound dressings.

International wound journal·2024
Same author

Enhancement Factors: A Central Concept during 50 Years of Surface-Enhanced Raman Spectroscopy.

ACS nano·2024
Same author

Generalised coupled-dipole model for core-satellite nanostructures.

Nanoscale·2023

Related Experiment Video

Updated: May 1, 2026

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

12.4K

Polypeptide multilayer self-assembly studied by ellipsometry.

Marina Craig1, Krister Holmberg2, Eric Le Ru3

  • 1Department of Chemical and Biological Engineering, Chalmers University of Technology, Sven Hultins Gata 12, 412 96 Gothenburg, Sweden ; Mölnlycke Health Care, P.O. Box 130 80, 402 52 Gothenburg, Sweden.

Journal of Drug Delivery
|March 25, 2014
PubMed
Summary

This study developed a polypeptide nanofilm for wound dressings. The novel material, built without a primer, shows potential as an infection-responsive bactericide release system for chronic wounds.

More Related Videos

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
09:54

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

6.8K
Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.7K

Related Experiment Videos

Last Updated: May 1, 2026

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

12.4K
Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
09:54

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

6.8K
Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Wound Healing

Background:

  • Chronic wounds are susceptible to bacterial infections, necessitating advanced treatment strategies.
  • Current wound dressings lack sophisticated infection-responsive drug delivery systems.
  • Nonwoven materials are widely used in wound care but require improved functionalities.

Purpose of the Study:

  • To develop an enzymatically degradable polypeptide nanofilm for chronic wound applications.
  • To create a novel lid for controlled release of bactericides triggered by infection levels.
  • To investigate the properties of a layer-by-layer self-assembled nanofilm on a nonwoven surface mimic.

Main Methods:

  • Layer-by-layer (LbL) self-assembly using Poly-L-lysine (PLL) and Poly-L-glutamic acid (PLGA).
  • Ellipsometry for characterizing nanofilm thickness in dry and wet states.
  • Enzyme adsorption studies using V8 (glutamyl endopeptidase) at varying temperatures.

Main Results:

  • A stable polypeptide nanofilm was successfully fabricated on a nonwoven mimic without a primer.
  • Nanofilm thickness reduced by 60% upon drying.
  • V8 enzyme adsorption was minimal at room temperature, suggesting stability in dry conditions.

Conclusions:

  • The PLL/PLGA nanofilm is a promising candidate for a primer-free, enzymatically degradable wound dressing component.
  • The nanofilm's stability at room temperature and potential for temperature-triggered degradation indicate suitability for infection-responsive drug delivery.
  • This technology could lead to improved therapeutic strategies for chronic wound infections.