Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staining kinetics in single cells. Part II. Diffusion processes inside the cell.

C Winzek1, H Baumgärtel

  • 1Institut für Physikalische und Theoretische Chemie, Freie Universität Berlin, Germany.

Histochemistry
|January 1, 1988
PubMed
Summary

This study investigated thionin dye uptake in cells and nuclei, finding that cell pretreatment and electrolyte concentration affect staining rates. A porous matrix model with adsorption explains the dye migration mechanism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The absorption spectra of anisole-h8, anisole-d3 and anisole-d8. The assignment of fundamental vibrations in the S0 and the S1 states.

Physical chemistry chemical physics : PCCP·2006
Same author

[Physician confidentiality].

Geburtshilfe und Frauenheilkunde·1994
Same author

[A spectroscopic study of the effect of the fixative media on dye adsorption by cells and tissues].

Acta histochemica·1988
Same author

[Can the metachromatic index be of help in cancer diagnosis?].

Acta histochemica·1988
Same author

Staining kinetics in single cells. Part I. Influence of convective diffusion on the staining rate.

Histochemistry·1988
Same author

Dissolution of calcified cholesterol stones and of brown and black pigment stones of the gallbladder.

Digestion·1988

Area of Science:

  • Cell biology
  • Biophysics
  • Spectroscopy

Background:

  • Understanding dye uptake mechanisms is crucial for cell analysis.
  • Thionin is a vital stain used in cell research.
  • Previous studies have not fully elucidated the kinetics of thionin dye migration within cells.

Purpose of the Study:

  • To investigate the time-dependent uptake of thionin in lymphocytes and monkey kidney cells.
  • To determine the factors influencing the rate of thionin staining.
  • To elucidate the mechanism of dye migration within cells using a spectroscopic approach.

Main Methods:

  • Spectroscopic measurement of time-dependent thionin uptake in lymphocytes, monkey kidney cells, and their nuclei.
  • Utilizing fixed cell material to eliminate plasma membrane and cytoplasm hindrance.

Related Experiment Videos

  • Applying hydrodynamic conditions to minimize convective diffusion effects.

    • Main Results:

    • Staining rate is dependent on thionin dye concentration, cell pretreatment, and electrolyte concentration.
    • Dye migration within cells follows a porous matrix model.
    • A dynamic, reversible equilibrium between migrating dye and pore wall binding sites, described by the Freundlich adsorption isotherm, was observed.

    Conclusions:

    • The proposed porous matrix model accurately explains the observed staining kinetics of thionin in cells.
    • Cellular pretreatment and solution electrolyte concentration are key modulators of thionin uptake.
    • Adsorption phenomena significantly influence dye diffusion within the cellular matrix.