Bioactive surface design based on functional composite electrospun nanofibers for biomolecule immobilization and...

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Nanomaterials and conducting polymers are key for enzyme immobilization matrices.
• Developing efficient immobilization matrices is crucial for biosensor performance.

Purpose of the Study:

• To design and investigate novel immobilization matrices for enzyme immobilization.
• To develop highly sensitive and stable glucose biosensors using modified nanofibers and conducting polymers.

Main Methods:

• Surface modification of graphite rod electrodes with electrospun nylon 6,6 nanofibers, with and without multiwalled carbon nanotubes (MWCNTs).
• Coating nanofibrous surfaces with a conducting polymer (PBIBA) to create electroactive immobilization matrices.
• Covalent immobilization of glucose oxidase onto the modified surfaces via aldehyde groups.

Main Results:

• Successful incorporation of MWCNTs into the nylon nanofiber matrix was confirmed.
• Electrodeposition of PBIBA protected the nanofibrous structure and enhanced protein attachment.
• The resulting glucose biosensors exhibited good operational stability, promising maximum current (Imax) values, and long shelf life.
• The biosensor was successfully applied for glucose detection in beverage samples.

Conclusions:

• The developed nylon-based nanofibrous matrices coated with PBIBA serve as effective immobilization platforms for glucose oxidase.
• The incorporation of MWCNTs further enhances biosensor performance, indicating potential for improved enzyme-based sensing applications.
• These novel glucose biosensors demonstrate stability and sensitivity suitable for practical applications, including food analysis.