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Dextran determination in human serum with the aid of an enzymatic glucose method
L F Gabel1, H E Kerkkamp, J N Nederstigt
1Institute of Anaesthesiology, Catholic University of Nijmegen, The Netherlands.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|October 1, 1988
Abstract:
A method is presented for the determination of dextran concentrations. Following acid hydrolysis and partial neutralization, the resulting glucose is determined with the aid of glucose oxidase. Specificity is better than that of previously applied methods. Mean recovery is 100.2 +/- 1.6% (SD) for concentrations from 180 mg.l-1 to 6000 mg.l-1. Coefficients of variation are 2.2% for 240 mg.l-1, 1.2% for 600 mg.l-1 and 1.8% for 1200 mg.l-1. When used for volume measurements, the accuracy is 99.3 +/- 2.1% (SD) for volumes of 2.5 to 3 liters.