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Updated: May 1, 2026

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Published on: April 24, 2018
The effects of the toxic cyanobacterium Limnothrix (strain AC0243) on Bufo marinus larvae
Olivia Daniels1, Larelle Fabbro2, Sandrine Makiela3
1School of Medical and Applied Sciences, Central Queensland University, Rockhampton 4701, Australia. o.daniels@cqu.edu.au.
Abstract:
Limnothrix (strain AC0243) is a cyanobacterium, which has only recently been identified as toxin producing. Under laboratory conditions, Bufo marinus larvae were exposed to 100,000 cells mL(-1) of Limnothrix (strain AC0243) live cultures for seven days. Histological examinations were conducted post mortem and revealed damage to the notochord, eyes, brain, liver, kidney, pancreas, gastrointestinal tract, and heart. The histopathological results highlight the toxicological impact of this strain, particularly during developmental stages. Toxicological similarities to β-N-Methylamino-L-alanine are discussed.

