Related Experiment Video
Updated: May 1, 2026
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Published on: October 8, 2015
[Detection of BRAF V600E mutation in hairy cell leukemia by high- resolution melting analysis]
Yanru Zhang1, Huimin Liu1, Junyuan Qi1
1Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.
Objective:
To explore the feasibility and diagnostic implication of BRAF V600E mutation identified by high-resolution melting (HRM) assay in patients with hairy cell leukemia (HCL).
Methods:
The V600E mutation of BRAF exon 15 in four HCL patients were detected by HRM assay and patients' clinical data were retrospectively analyzed.
Results:
All four HCL patients were positive for the BRAF V600E mutation, which were identical to the results of DNA sequencing.
Conclusion:
The HRM assay for BRAF V600E mutation provides a useful tool to aid the laboratory diagnosis of HCL with easy operability, accuracy, and low cost.
