Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles

Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 degrees C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre-stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.