Validation study of a commercial aflatoxin detection kit according to the notification method in Japan

A commercial aflatoxin detection ELISA kit, "RIDASCREEN(®) FAST Aflatoxin", was validated with corn samples naturally contaminated with aflatoxin and non-contaminated reference corn samples according to the Japanese Notification Method ShokuAnHatsu 0816-7. The trueness, intra-laboratory repeatability, intermediate precision, limit of detection and limit of quantitation were found to be 91%, 10%, 6.4%, 0.6μg/kg and 2μg/kg, respectively, and the performance of the kit was recognized as complying with all criteria in the Supplement Table of the Notification. These data suggest that this kit is useful as a simplified device to screen out negative corn samples contaminated with less than 4μg/kg.