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Updated: May 1, 2026
09:28
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Published on: May 18, 2020
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Model-based design and integration of a two-step biopharmaceutical production process
Bruno Otero1, Marcus Degerman, Thomas Budde Hansen
1Department of Chemical Engineering, Lund University, Box 124, 221 00, Lund, Sweden.
Bioprocess and Biosystems Engineering
|March 28, 2014
Summary
Simultaneous optimization of protein PEGylation and purification boosts productivity by 100% without sacrificing yield. Recycling unreacted protein increases yield by 30%, but batch mode is best for maximum productivity.
Area of Science:
- Biochemical Engineering
- Protein Modification
- Chromatographic Separation
Background:
- PEGylation is a crucial protein modification technique.
- Chromatographic purification is essential for isolating target PEGylated proteins.
- Separating mono-PEGylated proteins from unreacted and di-PEGylated forms presents challenges.
Purpose of the Study:
- To design and optimize a two-step process for protein PEGylation and purification.
- To compare the effects of separate versus simultaneous optimization of the PEGylation and purification steps.
- To investigate the impact of recycling unreacted protein on yield and productivity.
Main Methods:
- A two-step process involving protein PEGylation followed by chromatographic purification.
- Comparative analysis of separate and simultaneous optimization strategies.
- Evaluation of protein recycling strategies.
- Assessment of process productivity and yield.
Main Results:
- Simultaneous optimization increased productivity by up to 100% without reducing yield.
- Simultaneous optimization leverages the easier separation of di-PEGylated protein for improved yield and productivity.
- Recycling unreacted protein enhanced yield by 30%.
- Batch mode operation is preferred for maximizing productivity.
Conclusions:
- Simultaneous optimization of PEGylation and purification offers significant advantages over separate optimization.
- The process design effectively manages impurities like di-PEGylated proteins.
- Recycling strategies can improve overall yield, while batch mode maximizes productivity.
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