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A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Published on: April 2, 2012
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Herpes simplex virus growth, preparation, and assay
Peggy Marconi1, Roberto Manservigi
1Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy, mcy@unife.it.
Methods in Molecular Biology (Clifton, N.J.)
|March 28, 2014
Abstract:
In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.
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