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Related Experiment Video

Updated: May 1, 2026

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
06:14

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

6.6K

Herpes simplex virus growth, preparation, and assay.

Peggy Marconi1, Roberto Manservigi

  • 1Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy, mcy@unife.it.

Methods in Molecular Biology (Clifton, N.J.)
|March 28, 2014
PubMed
Summary

This chapter details laboratory procedures for growing herpes simplex virus (HSV) and preparing virus stocks. These methods are essential for studying HSV biology and its use in gene therapy applications.

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Area of Science:

  • Virology
  • Molecular Biology
  • Gene Therapy

Background:

  • Herpes simplex virus (HSV) research requires reliable virus stocks.
  • Understanding HSV biology is crucial for therapeutic development.
  • HSV vectors are promising tools for gene therapy.

Purpose of the Study:

  • To describe a specific laboratory protocol for HSV cultivation.
  • To provide a reproducible method for preparing HSV stocks.
  • To facilitate research in HSV biology and gene therapy.

Main Methods:

  • Detailed step-by-step procedures for virus propagation.
  • Methods for titration and quality control of virus stocks.
  • Guidelines for safe handling and storage of HSV.

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Related Experiment Videos

Last Updated: May 1, 2026

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
06:14

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

6.6K
Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

6.4K
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
12:22

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Published on: April 2, 2012

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Main Results:

  • Successful generation of high-titer HSV stocks.
  • Demonstration of protocol reproducibility.
  • Characterization of prepared virus stocks.

Conclusions:

  • The described protocol provides a reliable method for HSV stock preparation.
  • This method supports ongoing research in HSV biology.
  • The prepared stocks are suitable for gene therapy applications.