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Color standardization in whole slide imaging using a color calibration slide
Pinky A Bautista1, Noriaki Hashimoto1, Yukako Yagi1
1Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA.
Journal of Pathology Informatics
|March 28, 2014
Summary
Color calibration slides effectively normalize color variations in digital pathology images. This method significantly reduces color differences between whole slide scanners, improving consistency.
Area of Science:
- Digital pathology
- Histology imaging
- Color science
Background:
- Color consistency remains a challenge in digital pathology.
- Histological slide colors vary across different imaging systems.
Purpose of the Study:
- To address color variations in histology images.
- To normalize color differences between whole slide scanners.
Main Methods:
- Color correction utilized a color calibration slide with nine color patches.
- A color correction matrix was derived using spectral and scanned colors.
- Pixel colors were converted to target colors using the matrix coefficients.
Main Results:
- Significant reduction in CIELAB color difference (3.42 units, P < 0.001).
- Color differences between two whole slide scanners were substantially decreased.
- The method achieved a 95% confidence level for color correction.
Conclusions:
- Color calibration slides effectively normalize histological image color variations.
- Whole slide scanning-induced color variations can be normalized.
- This technique enhances color consistency in digital pathology.
