Coimmunoprecipitation of proteins from yeast
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Updated: May 1, 2026

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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Published on: May 3, 2015
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1Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.
Methods in Enzymology
|March 29, 2014
Abstract:
This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).
Keywords:
CoimmunoprecipitationImmunoprecipitationsNormalization of cell lysatesProtein–protein interactionsSchizosaccharomyces pombeSodium dodecyl sulfate polyacrylamide gel electrophoresisWestern blottingWhole cell lysatesMore Related Videos
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