Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Immunodeficiency Diseases01:25

Immunodeficiency Diseases

3.7K
Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency...
3.7K
Hematopoiesis01:21

Hematopoiesis

8.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trends and disparities in influenza and pneumonia associated sepsis mortality among older adults in the United States, 1999-2022: A retrospective population-based study.

Semergen·2026
Same author

[Exploring the protective effects and mechanisms of HYX1-derived exosomes on acute liver failure based on disulfidptosis signaling].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2026
Same author

TMAO Induced Kidney Aging by Activating ZBP1-Mediated Necroptosis.

Physiological research·2026
Same author

[Analysis on epidemiological characteristics of newly diagnosed occupational pneumoconiosis in Beijing from 2005 to 2021].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2026
Same author

Peripheral ameloblastoma with cervical lymph node metastasis: a case report and review of the literature.

International journal of oral and maxillofacial surgery·2026
Same author

[Economic burden of echinococcosis patients in endemic areas of western China: a meta-analysis].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2025
Same journal

The alternative NF-κB RelB subunit is a novel critical sensor of lipid metabolism to promote tumor development.

Cell death & disease·2026
Same journal

Activation of KIT signaling promotes early tumorigenesis through the AP-1 pathway in APC/TP53 double-knockout human colon organoids.

Cell death & disease·2026
Same journal

Rethinking LPCAT3 roles in human disease: broadening perspectives beyond ferroptosis.

Cell death & disease·2026
Same journal

GDPPH1 induces cell cycle arrest by downregulation of CDK4/Cyclin D1 via the m<sup>6</sup>A-METTL14-YTHDF2 axis, attenuating glioblastoma progression.

Cell death & disease·2026
Same journal

Tumor-derived PCSK9-enriched exosomes reprogram adipocytes to drive metabolic dysregulation and immune evasion in triple-negative breast cancer.

Cell death & disease·2026
Same journal

Disruption of MCL1/BOK transmembrane interaction as a novel strategy to induce cell death in tumours.

Cell death & disease·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Published on: October 3, 2019

11.3K

Cav-1 deletion impaired hematopoietic stem cell function.

L Bai1, G Shi1, L Zhang1

  • 1Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medical Center, Peking Union Medical College, Beijing 100021, China.

Cell Death & Disease
|March 29, 2014
PubMed
Summary
This summary is machine-generated.

Caveolin-1 (Cav-1) deficiency impairs hematopoietic stem cell (HSC) function by disrupting quiescence and differentiation. Cav-1-deficient mice show altered blood cell counts and reduced HSC self-renewal and reconstitution capacity.

More Related Videos

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

6.1K
Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

830

Related Experiment Videos

Last Updated: May 1, 2026

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Published on: October 3, 2019

11.3K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

6.1K
Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

830

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Hematopoietic stem cell (HSC) homeostasis relies on a delicate balance regulated by intrinsic and extrinsic factors.
  • Caveolin-1 (Cav-1), a protein in plasma membrane invaginations, is known to regulate neural and embryonic stem cell proliferation.
  • The specific role of Cav-1 in HSC function, particularly during aging, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of Cav-1 in hematopoietic stem cells (HSCs) during aging using Cav-1 knockout (Cav-1(-/-)) mice.
  • To elucidate the impact of Cav-1 deficiency on HSC number, cell cycle status, differentiation potential, and self-renewal capacity.

Main Methods:

  • Utilized Cav-1(-/-) mice to assess hematopoiesis and HSC function.
  • Employed Flow Cytometry (FACS) analysis to quantify HSC populations (LSKs, LT-HSCs, etc.).
  • Conducted in vitro clonogenic assays and in vivo competitive transplantation assays to evaluate HSC self-renewal and reconstitution potential.

Main Results:

  • Cav-1(-/-) mice exhibited altered blood cell composition (decreased B cells, increased M cells) due to increased HSC numbers, which worsened with age.
  • LSK, LT-HSC, and progenitor cell populations were significantly elevated in Cav-1(-/-) mice.
  • LT-HSCs from Cav-1(-/-) mice showed reduced self-renewal, disrupted quiescence, accelerated cell cycle progression (G2/M phase), and impaired differentiation capacity.

Conclusions:

  • Cav-1 deficiency impairs HSC quiescence and promotes cell cycle progression, leading to altered differentiation.
  • Cav-1 plays a critical role in maintaining HSC self-renewal and hematopoietic reconstitution.
  • Cav-1 deficiency induces environmental alterations that limit HSC function, highlighting its importance in hematopoiesis throughout aging.