Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LED-driven photo-Fenton process for micropollutant removal by nanostructured magnetite anchored in mesoporous silica.

Journal of environmental management·2023

Same author

Model-aided targeted volatile fatty acid production from food waste using a defined co-culture microbial community.

The Science of the total environment·2022

Same author

Microbial invasions in sludge anaerobic digesters.

Applied microbiology and biotechnology·2020

Same author

Steering the conversion of protein residues to volatile fatty acids by adjusting pH.

Bioresource technology·2020

Same author

Benchmarking environmental and economic indicators of sludge management alternatives aimed at enhanced energy efficiency and nutrient recovery.

Journal of environmental management·2020

Same author

Iron oxide-mediated photo-Fenton catalysis in the inactivation of enteric bacteria present in wastewater effluents at neutral pH.

Environmental pollution (Barking, Essex : 1987)·2020

Search research articles

Related Experiment Video

Updated: May 1, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

Solvent screening methodology for in situ ABE extractive fermentation.

H González-Peñas¹, T A Lu-Chau, M T Moreira

¹IFP Energies Nouvelles, Rond-point de l'échangeur de Solaize BP 3, 69360, Solaize, France, helena.gonzalez-penas@ifpen.fr.

Applied Microbiology and Biotechnology

|March 29, 2014

Summary

This summary is machine-generated.

Researchers screened solvents for acetone-butanol-ethanol (ABE) fermentation, identifying 2-butyl-1-octanol as the best for in situ extraction. This solvent enhanced butanol yield while remaining biocompatible with the fermentation bacteria.

More Related Videos

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Related Experiment Videos

Last Updated: May 1, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Area of Science:

Biotechnology
Chemical Engineering
Fermentation Technology

Background:

Acetone-butanol-ethanol (ABE) fermentation is a key bioprocess for producing valuable chemicals.
In situ product recovery is crucial for overcoming product inhibition and improving ABE fermentation efficiency.
Selecting appropriate solvents requires balancing extraction performance with biological compatibility.

Purpose of the Study:

To screen and evaluate various solvents for their efficacy in in situ liquid extraction of ABE fermentation products.
To assess the impact of selected solvents on biological parameters including biocompatibility, bioavailability, and product yield.
To identify optimal solvents that enhance n-butanol production and recovery from fermentation broth.

Main Methods:

Screening of 16 diverse chemical compounds for extraction capabilities in real ABE fermentation broth.
Evaluation of nine promising solvents for biocompatibility with Clostridium acetobutylicum.
Quantification of bioavailability, substrate consumption, and n-butanol production for biocompatible solvents.
Determination of partition coefficient and selectivity for assessing extraction performance.

Main Results:

2-butyl-1-octanol exhibited the highest partition coefficient (6.76) and significant selectivity (644).
The Guerbet alcohol 2-butyl-1-octanol led to the highest n-butanol yield (27.4%) among tested solvents.
This solvent demonstrated biocompatibility with Clostridium acetobutylicum, maintaining fermentation performance.
Solvents with higher affinity for butanol and butyric acid enhanced overall product yield.

Conclusions:

2-butyl-1-octanol is a highly effective solvent for in situ extraction in ABE fermentation.
The identified solvent enhances butanol yield and recovery while supporting microbial activity.
This study provides a robust methodology for solvent selection in bioprocessing applications.