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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Related Experiment Video

Updated: May 1, 2026

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors

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High-throughput cell aggregate culture for stem cell chondrogenesis.

Ahmad E Abu-Hakmeh¹, Leo Q Wan

¹Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2014

Summary

This study presents a 96-well plate method for creating stem cell aggregates, promoting chondrogenic differentiation. This high-throughput platform aids in optimizing conditions for cartilage tissue engineering.

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Last Updated: May 1, 2026

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors

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Published on: January 31, 2025

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Published on: April 27, 2017

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

Area of Science:

Stem cell biology
Tissue engineering
Biotechnology

Background:

Cell aggregate culture is a reliable method for stem cell chondrogenesis.
High-throughput cell pellet culture facilitates screening of factors affecting stem cell function.
Mesenchymal condensation is a key process in chondrogenesis.

Purpose of the Study:

To develop a high-throughput platform for stem cell aggregate formation in a 96-well plate.
To optimize conditions for chondrogenic differentiation of stem cells in aggregates.
To facilitate screening of soluble factors for cartilage tissue engineering.

Main Methods:

Stem cells are centrifuged to form high-density pellets in a 96-well plate.
Pellets mimic mesenchymal condensation.
Aggregates are cultured in chondrogenic medium for 4 weeks.

Main Results:

A reproducible platform for stem cell aggregate formation was established.
The method allows for efficient chondrogenic differentiation of stem cells.
The system is compatible with high-throughput screening.

Conclusions:

The 96-well plate platform enables high-throughput screening of factors influencing stem cell chondrogenesis.
This technique is valuable for optimizing cartilage tissue engineering strategies.
The method provides a reliable system for studying stem cell differentiation.