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Related Experiment Video

Updated: May 1, 2026

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

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Configurable low-cost plotter device for fabrication of multi-color sub-cellular scale microarrays.

Giuseppe Arrabito1, Hendrik Schroeder, Kathrin Schröder

  • 1TU Dortmund, Fakultät für Chemie und Chemische Biologie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Str. 6, 44227, Dortmund, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|March 29, 2014
PubMed
Summary

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A low-cost plotter enables the fabrication of high-density DNA microarrays for multiplexed single-cell analyses. These biochips facilitate cell biology research by concentrating and activating epidermal growth factor receptors (EGFR) in living cells.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Multiplexed single-cell analyses require high-density microarrays.
  • Fabricating such arrays can be costly and complex.
  • Accessible tools are needed for academic research.

Purpose of the Study:

  • To report the construction and operation of a low-cost plotter for microarray fabrication.
  • To demonstrate the plotter's capability for creating DNA microarrays for cell biological studies.
  • To enable fundamental research in cell signaling at subcellular resolution.

Main Methods:

  • A plotter was constructed using polymeric pyramidal pens and a rotation stage.
  • Microarrays were printed on glass substrates using a Lab-View controlled interface.
Keywords:
DNA-directed protein immobilizationEGF receptorsdevice automationmultiplexed patternspolymer pen lithography

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Last Updated: May 1, 2026

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  • DNA microarrays were functionalized with epidermal growth factor peptides via DNA-directed immobilization.

    • Main Results:

    • The plotter, assembled for under 15,000 Euro, produces DNA microarrays with feature sizes of 5 μm and densities up to 10(5) spots/mm2.
    • Fabricated DNA microarrays enabled the creation of sub-cellular scale arrays of bioactive peptides.
    • Biochips demonstrated specific recruitment, concentration, and activation of epidermal growth factor receptors in living cells.

    Conclusions:

    • The low-cost plotter provides access to bio-functionalized arrays for fundamental cell biology research.
    • The technology supports the manipulation of cellular signal pathways at subcellular resolution.
    • This work advances accessible tools for advanced cell imaging and analysis.