Activity-based proteomics probes for carbohydrate-processing enzymes: current trends and future outlook.

Area of Science:

• Biochemistry
• Glycobiology
• Proteomics

Background:

• Carbohydrate-processing enzymes play crucial roles in biological processes, influencing health and disease.
• Understanding these enzymes is vital due to their involvement in complex glycan structure formation and breakdown.
• Identifying these enzymes in biological systems presents a significant challenge.

Purpose of the Study:

• To review the design principles of activity-based proteomics probes (ABPPs).
• To highlight the application of ABPPs in profiling carbohydrate-processing enzyme activity.
• To discuss future perspectives for ABPPs in glycobiology.

Main Methods:

• Activity-based proteomics probes (ABPPs) are chemical tools designed to covalently label active enzymes.
• ABPPs enable the detection and identification of enzyme activity within complex biological samples.
• This review focuses on the design and application of ABPPs specifically for carbohydrate-processing enzymes.

Main Results:

• ABPPs have emerged as powerful tools for detecting and identifying functionally related carbohydrate-processing enzymes.
• Examples of ABPPs used to profile enzyme activity in glycobiology are presented.
• The review provides insights into the current landscape and potential of ABPPs in this field.

Conclusions:

• Activity-based proteomics probes are effective for studying carbohydrate-processing enzymes.
• ABPPs facilitate the identification of enzymes involved in glycan metabolism, relevant to health and disease.
• Further development and application of ABPPs hold promise for advancing glycobiology research.