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Updated: Jul 12, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
Structure and gene cluster of the capsular polysaccharide of Acinetobacter baumannii K205
Andrei V Filatov1, Andrei V Perepelov1, Anna M Shpirt1
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
Structure of the Acinetobacter baumannii strain MAR22-3311 classified to KL205 type capsular polysaccharide (CPS) was established by component analyses, Smith degradation as well as 1H and 13C NMR spectroscopy, including two-dimensional homonuclear 1Н,1Н COSY, TOCSY, ROESY and heteronuclear 1Н,13C HSQC and HMBC experiments. The K205 CPS is composed of branched hexasaccharide K-units containing three d-GalNAc residues, and one residue each of d-Quip3NAc, d-Galp and d-Glcp. The following structure of the hexasaccharide repeating unit of the CPS was established. Functions of genes in the K locus of A. baumannii MAR22-3311 (K205) were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.
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