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Updated: Jun 12, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
Structure and gene cluster of the K166 capsular polysaccharide from Acinetobacter baumannii MAR 21-5059
Evelina L Zdorovenko1, Andrey S Dmitrenok1, Mikhail M Shneider2
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
The structure of capsular polysaccharide (CPS) classified to K166 of Acinetobacter baumannii MAR 21-5059 strain was established using component analyses as well as 1H and 13C NMR spectroscopy, including two-dimensional homonuclear 1Н,1Н COSY, TOCSY, ROESY and heteronuclear 1Н,13C HSQC, HMBC, HSQC-TOCSY and HSQC-NOESY experiments. The CPS is composed of branched tetrasaccharide K-units containing two d-GlcNAc residues, one d-Gal and rarely occurred residue, l-RhaNAc3NAc. The following structure of the repeating K-unit was established: To our knowledge the СPS structure is novel and has not been previously published. Functions of genes in the KL166 locus of A. baumannii MAR 21-5059 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.
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