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T cells recognize both conformational and cryptic determinants on the insulin molecule
J W Thomas1, H George-Gattner, W Danho
1Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
T lymphocytes from strain 2 guinea pigs were studied for recognition of isolated insulin A and B chains. Insulin-immune T cells require intact conformation of the interchain disulfide bond that forms the A chain loop but do not require the B chain. In addition, strain 2 T cells were unexpectedly found to recognize unique epitopes on isolated insulin B chain that are not generated by presentation of native insulin. These data show that the T cell repertoire of strain 2 guinea pig for insulin includes both conformational and cryptic determinants.
T lymphocytes from strain 2 guinea pigs were studied for recognition of isolated insulin A and B chains. Insulin-immune T cells require intact conformation of the interchain disulfide bond that forms the A chain loop but do not require the B chain. In addition, strain 2 T cells were unexpectedly found to recognize unique epitopes on isolated insulin B chain that are not generated by presentation of native insulin. These data show that the T cell repertoire of strain 2 guinea pig for insulin includes both conformational and cryptic determinants.