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Electrophoretic location of epitopes on human transferrin
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Competitive assays of antigen--antibody interaction using solid-bound antigen or antibodies can be affected by steric restrictions. A suitable electrophoretic system may be used to follow the interactions in solution. Electrophoresis of human transferrin complexed with pairs of monoclonal antibodies raised against it revealed three types of interaction: competition for closely neighbouring epitopes, simultaneous binding to distinct epitopes, and a type of interaction suggesting formation of multiply linked antigen--antibody complexes.
Competitive assays of antigen--antibody interaction using solid-bound antigen or antibodies can be affected by steric restrictions. A suitable electrophoretic system may be used to follow the interactions in solution. Electrophoresis of human transferrin complexed with pairs of monoclonal antibodies raised against it revealed three types of interaction: competition for closely neighbouring epitopes, simultaneous binding to distinct epitopes, and a type of interaction suggesting formation of multiply linked antigen--antibody complexes.